4GT6

Crystal structure of a leucine rich cell surface protein (FAEPRAA2165_01021) from Faecalibacterium prausnitzii A2-165 at 1.80 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-02-28
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 65
Unit cell lengths	119.538, 119.538, 77.568
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.927 - 1.800
R-factor	0.1517
Rwork	0.151
R-free	0.17410
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.070
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.20)
Phasing software	SHELX
Refinement software	BUSTER-TNT (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	26.927	26.927	1.850
High resolution limit [Å]	1.800	8.050	1.800
Rmerge		0.069	0.732
Total number of observations		5790	30272
Number of reflections	58422
<I/σ(I)>	11	23.8	2.7
Completeness [%]	99.9	97.4	99.9
Redundancy	7.7	8.5	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	277	30.00% polyethylene glycol 6000, 0.1M Bicine pH 9.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K