4GT5
Crystal structure of the inactive TrkA kinase domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2011-03-13 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.03321 |
Spacegroup name | H 3 2 |
Unit cell lengths | 105.033, 105.033, 203.316 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.384 - 2.398 |
R-factor | 0.2039 |
Rwork | 0.202 |
R-free | 0.24790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1luf |
RMSD bond length | 0.004 |
RMSD bond angle | 0.758 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.460 |
High resolution limit [Å] | 2.398 | 2.398 |
Number of reflections | 17203 | |
<I/σ(I)> | 52.9 | 5.3 |
Completeness [%] | 99.8 | 100 |
Redundancy | 11.1 | 11.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 294.15 | 1.5 M sodium chloride, 0.1 M MES, pH 6.5, 0.2 M sodium/potassium phosphate, VAPOR DIFFUSION, HANGING DROP, temperature 294.15K |