4GSV
Crystal Structure of the Ni2+2-Human Arginase I-ABH complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-12-07 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0750 |
Spacegroup name | P 3 |
Unit cell lengths | 90.711, 90.711, 69.658 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 1.480 |
R-factor | 0.1531 |
Rwork | 0.153 |
R-free | 0.19250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2pha |
RMSD bond length | 0.007 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.530 |
High resolution limit [Å] | 1.480 | 1.480 |
Rmerge | 0.067 | 0.354 |
<I/σ(I)> | 21.588 | 4.362 |
Completeness [%] | 99.8 | 100 |
Redundancy | 4.7 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | A metal-free HAI crystal was soaked in 5 mM NiCl2, 5 mM ABH, 100 mM HEPES (pH 7.0), 32% (w/v) Jeffamine ED-2001 for 26 hours., VAPOR DIFFUSION, SITTING DROP, temperature 298K |