4GSA
CRYSTAL STRUCTURE OF GLUTAMATE-1-SEMIALDEHYDE AMINOMUTASE (AMINOTRANSFERASE) REDUCED WITH CYANOBOROHYDRATE
Experimental procedure
Source type | ROTATING ANODE |
Source details | ELLIOTT GX-21 |
Temperature [K] | 273 |
Detector technology | IMAGE PLATE |
Collection date | 1995-04-07 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 68.640, 108.280, 122.440 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.500 |
R-factor | 0.187 |
Rwork | 0.187 |
R-free | 0.26400 |
Starting model (for MR) | GSAT WILD-TYPE STRUCTURE |
RMSD bond length | 0.011 |
RMSD bond angle | 1.850 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.630 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.073 * | |
Total number of observations | 91742 * | |
Number of reflections | 31141 | |
<I/σ(I)> | 9.7 | 2 |
Completeness [%] | 96.8 | 91.7 |
Redundancy | 2.9 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 7 | 4 * | Hennig, M., (1994) J.Mol.Biol., 242, 591. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 3.6-6 (mg/ml) | |
2 | 1 | drop | sodium cacodylate | 30 (mM) | |
3 | 1 | drop | magnesium acetate | 80 (mM) | |
4 | 1 | drop | PEG10000 | 7.8 (%) | |
5 | 1 | reservoir | magnesium acetate | 200 (mM) | |
6 | 1 | reservoir | PEG10000 | 19.5 (%) |