4GS3

Dimeric structure of the N-terminal domain of PriB protein from Thermoanaerobacter tencongensis solved ab initio

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2012-02-06
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.000
Spacegroup name	C 1 2 1
Unit cell lengths	55.560, 41.180, 38.780
Unit cell angles	90.00, 94.80, 90.00

Refinement procedure

Resolution	30.000 - 1.090
R-factor	0.1292
Structure solution method	AB INITIO PHASING
RMSD bond length	0.016
RMSD bond angle	2.800
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	SHELXD
Refinement software	SHELXL-97

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.090
Rmerge	0.047
Number of reflections	38376
<I/σ(I)>	29.3
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	0.14 M ammonium acetate, 0.07 M acetate buffer pH 4.6, 21% PEG 4K, VAPOR DIFFUSION, HANGING DROP, temperature 293K