4GS2
DNA Holliday junction stabilized by iodine halogen bond. I1J construct in related reference.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 133 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-10-31 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 65.739, 25.187, 37.171 |
| Unit cell angles | 90.00, 110.88, 90.00 |
Refinement procedure
| Resolution | 23.300 - 1.900 |
| Rwork | 0.235 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2org |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.900 |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 3432 |
| Completeness [%] | 73.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 7 | 298 | 0.7mM DNA, 25mM sodium cacodylate pH 7.0 buffer, 10-25mM calcium chloride, and 0.8-1.2mM spermine, equilibrated against a reservoir of 30-40% aqueous MPD, hanging drop, temperature 298K |
