4GRH
Crystal structure of pabB of Stenotrophomonas maltophilia
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0809 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 161.300, 161.300, 116.089 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.570 - 2.250 |
| R-factor | 0.19633 |
| Rwork | 0.194 |
| R-free | 0.24038 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k0g |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.159 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 46.600 |
| High resolution limit [Å] | 2.250 |
| Rmerge | 0.062 |
| Number of reflections | 42573 |
| <I/σ(I)> | 20.1 |
| Completeness [%] | 100.0 |
| Redundancy | 42.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 295 | 0.05 M magnesium chloride, 0.1 M HEPES, 12-15% w/v PEG550 MME, 0.1% CHAPS, pH 7.8, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
