4GR7
The human W42R Gamma D-Crystallin Mutant Structure at 1.7A Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2012-05-04 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.54 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 68.607, 68.607, 133.382 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.500 - 1.700 |
R-factor | 0.20224 |
Rwork | 0.200 |
R-free | 0.24458 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hk0 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.081 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.500 | 1.740 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.574 | |
Completeness [%] | 92.1 | 84 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.18 M Li2SO4, 0.1 M Tris-HCl, 22% (w/v) PEG 4000, 15% (v/v) glycerol , pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |