4GQ7
Crystal structure of Lg-Flo1p
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-27 |
| Detector | BRUKER SMART 1000 |
| Wavelength(s) | 0.968 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.056, 59.947, 82.344 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 9.990 - 2.530 |
| R-factor | 0.1888 |
| Rwork | 0.186 |
| R-free | 0.23980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xjv |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.020 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 9.990 |
| High resolution limit [Å] | 2.530 |
| Rmerge | 0.049 |
| Number of reflections | 6042 |
| <I/σ(I)> | 5.9 |
| Completeness [%] | 97.9 |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 295 | 25% w/v PEG4000, 0.05 M potassium phosphate monobasic, VAPOR DIFFUSION, temperature 295K |
