4GQ1
Nup37 of S. pombe
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.075 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 131.026, 131.026, 116.948 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.000 - 2.400 |
R-factor | 0.18476 |
Rwork | 0.184 |
R-free | 0.20475 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.408 |
Data reduction software | HKL-2000 |
Data scaling software | SADABS |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 2.500 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 38275 | |
Completeness [%] | 100.0 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 7.4 | 303 | pH 7.4, EVAPORATION, temperature 303K |