4GPT

Crystal structure of KPT251 in complex with CRM1-Ran-RanBP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Detector technology	CCD
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	105.941, 105.941, 305.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.220
R-factor	0.16698
Rwork	0.165
R-free	0.20984
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.180
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CCP4
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall
Low resolution limit [Å]	100.093
High resolution limit [Å]	2.220
Number of reflections	85858
Completeness [%]	99.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.6	293	pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K