4GPS
Crystal Structure of K. lactis Dxo1 (YDR370C)
Experimental procedure
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979, 0.9793, 0.9724 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 82.820, 82.820, 259.850 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.930 - 2.400 |
R-factor | 0.197 |
Rwork | 0.197 |
R-free | 0.24000 |
Structure solution method | MAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.700 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.400 |
Number of reflections | 20835 |
Completeness [%] | 95.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2M Sodium citrate tribasic, 23.5% (w/v) PEG 3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |