4GPG
X/N joint refinement of Achromobacter Lyticus Protease I free form at pD8.0
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | NUCLEAR REACTOR |
Source details | JRR-3M BEAMLINE 1G-C |
Synchrotron site | JRR-3M |
Beamline | 1G-C |
Temperature [K] | 297 |
Detector technology | IMAGE PLATE |
Collection date | 2007-06-12 |
Wavelength(s) | 2.6 |
Spacegroup name | P 1 |
Unit cell lengths | 39.574, 40.785, 43.980 |
Unit cell angles | 64.82, 66.10, 73.42 |
Refinement procedure
Resolution | 37.610 - 1.979 |
R-factor | 0.1984 |
Rwork | 0.195 |
R-free | 0.25950 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.473 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | PHENIX ((phenix.refine: 1.7.3_911)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.950 |
High resolution limit [Å] | 1.980 | 4.310 | 1.880 |
Rmerge | 0.161 | 0.124 | 0.198 |
Number of reflections | 11000 | ||
<I/σ(I)> | 4.87 | 10.7 | 5.95 |
Completeness [%] | 89.4 | 98.1 | 89.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 297 | 20% w/v PEG 3350, 10mM Tris, HCl pH 8.0, 50% PEG 3350, HCl pD 8.0, VAPOR DIFFUSION, temperature 297K |
1 | VAPOR DIFFUSION | 8 | 297 | 20% w/v PEG 3350, 10mM Tris, HCl pH 8.0, 50% PEG 3350, HCl pD 8.0, VAPOR DIFFUSION, temperature 297K |