4GP5

Structure of Recombinant Cytochrome ba3 Oxidase mutant Y133W from Thermus thermophilus

Replaces:  4EV3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	144.090, 97.960, 94.650
Unit cell angles	90.00, 127.99, 90.00

Refinement procedure

Resolution	74.600 - 2.700
R-factor	0.17621
Rwork	0.173
R-free	0.22993
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.563
Data reduction software	HKL-3000
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall
Low resolution limit [Å]	74.600
High resolution limit [Å]	2.700
Number of reflections	28621
Completeness [%]	97.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	6.5	293	50mM Sodium Cacodylate, 1.6M NaCl, 40% PEG400, pH 6.5, Lipidic Cubic Phase, temperature 293K