4GOX
Sulfotransferase Domain from the Synechococcus PCC 7002 Olefin Synthase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-15 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97934 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 131.440, 131.440, 47.080 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.070 - 2.150 |
R-factor | 0.1898 |
Rwork | 0.188 |
R-free | 0.21970 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.060 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.230 |
High resolution limit [Å] | 2.150 | 4.630 | 2.150 |
Rmerge | 0.035 | 0.633 | |
Number of reflections | 23099 | ||
Completeness [%] | 99.8 | 98.4 | 100 |
Redundancy | 8 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 27-30% PEG1500, 100 mM MMT buffer (DL-malic acid, MES, Tris), pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |