4GOW
Crystal Structure of Ca2+/CaM:Kv7.4 (KCNQ4) B helix complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Detector technology | CCD |
Wavelength(s) | 1.006 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 104.075, 104.075, 113.729 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.093 - 2.600 |
R-factor | 0.2379 |
Rwork | 0.236 |
R-free | 0.26820 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.790 |
Data reduction software | MOSFLM (3.3.9) |
Data scaling software | SCALA (3.3.9) |
Phasing software | PHASER |
Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 90.131 | 113.730 | 2.740 |
High resolution limit [Å] | 2.600 | 8.220 | 2.600 |
Rmerge | 0.041 | 0.965 | |
Number of reflections | 11737 | ||
<I/σ(I)> | 18.9 | 14.3 | 0.8 |
Completeness [%] | 100.0 | 99.6 | 100 |
Redundancy | 16.7 | 14.2 | 12.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 1.6M ammonium sulfate, 0.2M sodium citrate (pH 6.0), 0.1M sodium tartrate, 4% isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |