4GOU

Crystal structure of an RGS-RhoGEF from Entamoeba histolytica

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2010-10-11
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.97954
Spacegroup name	C 1 2 1
Unit cell lengths	86.124, 46.259, 142.637
Unit cell angles	90.00, 104.20, 90.00

Refinement procedure

Resolution	46.092 - 2.300
R-factor	0.1853
Rwork	0.182
R-free	0.23570
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.080
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (AutoSol)
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.320
High resolution limit [Å]	2.300	2.300
Rmerge	0.089	0.584
Number of reflections	46832
<I/σ(I)>	18.5	2
Completeness [%]	98.8	86.4
Redundancy	4.3	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	291	EhRGS-RhoGEF at 13 mg/mL in 50 mM HEPES pH 7.5, 100 mM NaCl, and 5 mM DTT was mixed 1:1 with and equilibrated against crystalization solution (16% w/v PEG 3350 and 100 mM sodium malonate pH 5.0), VAPOR DIFFUSION, HANGING DROP, temperature 291K