4GOJ

The Crystal Structure of full length Arl3GppNHp in complex with UNC119a

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Detector technology	CCD
Collection date	2012-02-29
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	69.750, 103.300, 119.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.970 - 2.100
R-factor	0.21533
Rwork	0.214
R-free	0.24832
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bh6 3gqq
RMSD bond length	0.008
RMSD bond angle	1.147
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.6.0093)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.970	2.200
High resolution limit [Å]	2.100	2.100
Rmerge	0.098	0.395
Number of reflections	51107
<I/σ(I)>	13.06
Completeness [%]	99.6	99.7
Redundancy		5.53

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	3.4 M Sodium Formate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K