4GOC
Crystal structure of the Get5 ubiquitin-like domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-02-26 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.360, 63.620, 71.580 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.069 - 2.400 |
R-factor | 0.1809 |
Rwork | 0.174 |
R-free | 0.24530 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.103 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8_1066)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.070 | 2.460 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.133 | 0.613 |
Number of reflections | 9092 | |
<I/σ(I)> | 6.6 | 1.8 |
Completeness [%] | 98.2 | 98.7 |
Redundancy | 3.3 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 2.4 M sodium malonate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |