4GO8
Crystal Structure of the TREHALULOSE SYNTHASE MUTB, MUTANT A258V, in complex with TRIS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97559 |
| Spacegroup name | P 1 |
| Unit cell lengths | 63.510, 71.310, 81.530 |
| Unit cell angles | 68.04, 72.72, 70.10 |
Refinement procedure
| Resolution | 14.820 - 2.150 |
| R-factor | 0.194 |
| Rwork | 0.190 |
| R-free | 0.24950 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1zjb |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.665 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.820 | 2.300 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.314 | |
| Number of reflections | 62687 | |
| <I/σ(I)> | 7.6 | 3.46 |
| Completeness [%] | 94.0 | 93.3 |
| Redundancy | 1.9 | 1.86 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 292 | 0.1M Tris-HCl, 12%(w/v) PEG20000, 0.01M L-Cysteine, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
