4GO1
Crystal Structure of full length transcription repressor LsrR from E. coli.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-09-22 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.9999 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 79.702, 113.866, 185.271 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.000 |
R-factor | 0.21969 |
Rwork | 0.216 |
R-free | 0.26759 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2w48 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.319 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.050 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.092 | 0.283 |
Number of reflections | 17219 | |
<I/σ(I)> | 21.2 | 5.2 |
Completeness [%] | 99.5 | 95.1 |
Redundancy | 7.3 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 0.1M Hepes, 6%(w/v) PEG8000, 8%(v/v) ethylene glycol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |