4GNM
Structure of rat cytosolic PEPCK Ld_2g in complex with oxalate and GTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-01-31 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.491, 84.706, 118.775 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.660 - 1.500 |
R-factor | 0.18955 |
Rwork | 0.188 |
R-free | 0.22179 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3dt2 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.662 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 100.000 | 1.550 |
High resolution limit [Å] | 1.500 | 3.230 | 1.500 |
Rmerge | 0.112 | 0.097 | 0.987 |
Number of reflections | 94980 | ||
<I/σ(I)> | 10.6 | ||
Completeness [%] | 96.3 | 91.6 | 96.5 |
Redundancy | 4.4 | 4.5 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 22-26% PEG3350, 0.1 M HEPES, pH 7.4, 10 mM manganese chloride, 10 mM GTP, VAPOR DIFFUSION, HANGING DROP, temperature 298K |