4GNI
Structure of the Ssz1 ATPase bound to ATP and Magnesium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-04 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.87 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 80.630, 55.380, 98.790 |
Unit cell angles | 90.00, 106.53, 90.00 |
Refinement procedure
Resolution | 36.066 - 1.796 |
R-factor | 0.2021 |
Rwork | 0.201 |
R-free | 0.24360 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.107 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.070 | 1.890 |
High resolution limit [Å] | 1.796 | 1.796 |
Rmerge | 0.104 | |
Number of reflections | 78273 | |
<I/σ(I)> | 9 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.1 M HEPES, pH 7.5; 20 % PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 291K |