4GMN
Structural basis of Rpl5 recognition by Syo1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9768 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 79.620, 104.230, 182.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.239 - 2.950 |
| R-factor | 0.2088 |
| Rwork | 0.203 |
| R-free | 0.25920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.259 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.270 | 3.110 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.127 | 0.441 |
| Number of reflections | 16385 | |
| <I/σ(I)> | 11.2 | 4.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.1 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 0.1 M succinic acid pH 7.0, 20% (v/v) PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
