4GM9
Crystal structure of human WD repeat domain 5 with compound MM-401
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-02-25 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97857 |
Spacegroup name | P 1 |
Unit cell lengths | 46.904, 47.242, 68.896 |
Unit cell angles | 88.50, 89.50, 74.53 |
Refinement procedure
Resolution | 37.822 - 2.100 |
R-factor | 0.1613 |
Rwork | 0.159 |
R-free | 0.20780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2h14 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.919 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 100.000 | 2.180 |
High resolution limit [Å] | 2.100 | 4.520 | 2.100 |
Number of reflections | 32452 | ||
<I/σ(I)> | 14.2 | 3.8 | |
Completeness [%] | 97.8 | 97.7 | 94.9 |
Redundancy | 1.9 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 30% PEG4000, 0.2 M magnesium chloride, 0.1 M Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.00K |