4GM3
Crystal structure of human WD repeat domain 5 with compound MM-101
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-25 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 1 |
Unit cell lengths | 48.943, 105.985, 120.907 |
Unit cell angles | 90.00, 89.76, 90.03 |
Refinement procedure
Resolution | 48.536 - 3.393 |
R-factor | 0.1968 |
Rwork | 0.195 |
R-free | 0.22580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2h14 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.576 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 100.000 | 3.520 |
High resolution limit [Å] | 3.393 | 7.320 | 3.393 |
Number of reflections | 30393 | ||
<I/σ(I)> | 7.9 | 4.5 | |
Completeness [%] | 90.5 | 98.8 | 68.9 |
Redundancy | 1.9 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 25% PEG4000, 0.2 M ammonium sulfate, 0.1 M sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |