4GLM
Crystal structure of the SH3 Domain of DNMBP protein [Homo sapiens]
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-07-29 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97931 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 58.279, 136.203, 33.623 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.820 - 1.900 |
R-factor | 0.2302 |
Rwork | 0.229 |
R-free | 0.25870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2drk |
Data scaling software | SCALEPACK |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.930 |
High resolution limit [Å] | 1.900 | 5.160 | 1.900 |
Rmerge | 0.080 | 0.031 | 0.757 |
Number of reflections | 21923 | ||
<I/σ(I)> | 9 | ||
Completeness [%] | 99.9 | 99.4 | 100 |
Redundancy | 7.5 | 7.1 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 291 | 5% PEG 8000, 0.2 M NaCl, and 0.1 M Tris 8.5, vapor diffusion hanging drop, temperature 291K |