4GLI
Crystal Structure of Human SMN YG-Dimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2010-02-11 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 87.745, 65.914, 84.908 |
Unit cell angles | 90.00, 110.48, 90.00 |
Refinement procedure
Resolution | 26.734 - 1.903 |
R-factor | 0.20533 |
Rwork | 0.203 |
R-free | 0.24709 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1omp |
RMSD bond length | 0.024 |
RMSD bond angle | 1.982 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.734 | |
High resolution limit [Å] | 1.903 | 1.903 |
Rmerge | 0.126 | 0.560 |
Number of reflections | 35714 | |
<I/σ(I)> | 19.7 | 2.4 |
Completeness [%] | 99.4 | 98.8 |
Redundancy | 5.3 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 294.15 | 0.1 M Tris-HCl, pH 8.0, 14% w/v PEG3350, 10 mM calcium chloride, 0.1 M potassium chloride, 0.1 M ammonium sulfate, 18% ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 294.15K |