4GLG
DNA dodecamer containing 5-methyl cytosine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-06-09 |
Detector | DECTRIS PILATUS 2M-F |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 24.744, 41.885, 66.062 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.900 - 1.720 |
R-factor | 0.22 |
Rwork | 0.220 |
R-free | 0.28700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dpn |
RMSD bond length | 0.009 |
RMSD bond angle | 0.030 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.920 | 1.820 |
High resolution limit [Å] | 1.720 | 1.720 |
Rmerge | 0.024 | 0.213 |
Number of reflections | 7618 | |
<I/σ(I)> | 28.99 | 6.94 |
Completeness [%] | 97.9 | 96.1 |
Redundancy | 3.65 | 3.75 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 298 | 0.04M sodium cacodylate buffer, 0.08M sodium chloride, 0.012M spermine tetra hydrochloride, 27% MPD, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |