4GLG
DNA dodecamer containing 5-methyl cytosine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-06-09 |
| Detector | DECTRIS PILATUS 2M-F |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 24.744, 41.885, 66.062 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.900 - 1.720 |
| R-factor | 0.22 |
| Rwork | 0.220 |
| R-free | 0.28700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dpn |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.030 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.920 | 1.820 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.024 | 0.213 |
| Number of reflections | 7618 | |
| <I/σ(I)> | 28.99 | 6.94 |
| Completeness [%] | 97.9 | 96.1 |
| Redundancy | 3.65 | 3.75 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 298 | 0.04M sodium cacodylate buffer, 0.08M sodium chloride, 0.012M spermine tetra hydrochloride, 27% MPD, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
