4GLF
Crystal structure of methylthioadenosine phosphorylase sourced from an antarctic soil metagenomic library
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-26 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 63 |
Unit cell lengths | 80.648, 80.648, 81.249 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 1.980 |
R-factor | 0.15346 |
Rwork | 0.151 |
R-free | 0.20884 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1v4n |
RMSD bond length | 0.020 |
RMSD bond angle | 1.894 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.050 |
High resolution limit [Å] | 1.980 | 1.980 |
Rmerge | 0.086 | 0.375 |
Number of reflections | 20266 | |
<I/σ(I)> | 26.92 | 2.8 |
Completeness [%] | 96.6 | 74.5 |
Redundancy | 11.5 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 30%PPG400, 75mM MgCl2, BIS-TRIS, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |