4GLC
DNA dodecamer containing 5-hydroxymethyl-cytosine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-06-09 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 24.513, 41.602, 66.044 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.200 - 1.831 |
| R-factor | 0.233 |
| Rwork | 0.233 |
| R-free | 0.30600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dpn |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.028 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.600 | 1.940 |
| High resolution limit [Å] | 1.831 | 1.831 |
| Rmerge | 0.034 | 0.216 |
| Number of reflections | 6201 | |
| <I/σ(I)> | 24.06 | 6.65 |
| Completeness [%] | 97.5 | 96.6 |
| Redundancy | 3.68 | 3.62 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 293 | 0.04M sodium cacodylate buffer, 0.08M sodium chloride, 0.012M spermine tetra hydrochloride, 30% MPD, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
