4GKQ
Structure of the neck and C-terminal motor homology domain of ViK1 from Candida glabrata
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-15 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.033 |
Spacegroup name | H 3 |
Unit cell lengths | 197.000, 197.000, 50.180 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.800 - 2.990 |
R-factor | 0.2182 |
Rwork | 0.214 |
R-free | 0.25940 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.160 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.070 | |
High resolution limit [Å] | 2.990 | 13.370 | 2.990 |
Rmerge | 0.088 | 0.072 | 0.928 |
Number of reflections | 14501 | 72 | 1076 |
<I/σ(I)> | 13.14 | 17.99 | 2.13 |
Completeness [%] | 98.8 | 43.1 | 100 |
Redundancy | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 0.1M Tris, 12% PEG8000, 0.05M MgCl2, 0.15M NaCl, 5% Ethylene glycol, 1mM TCEP, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |