4GKP
Structure of the truncated neck and C-terminal motor homology domain of ViK1 from Candida glabrata
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-05 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.976 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.742, 77.722, 107.737 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.830 - 2.420 |
R-factor | 0.212 |
Rwork | 0.209 |
R-free | 0.25930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2o0a |
RMSD bond length | 0.009 |
RMSD bond angle | 1.255 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 20.000 | 2.500 |
High resolution limit [Å] | 2.420 | 10.000 | 2.420 |
Rmerge | 0.035 | 0.025 | 0.193 |
Number of reflections | 21752 | 272 | 1880 |
<I/σ(I)> | 37.65 | 69.06 | 8.17 |
Completeness [%] | 98.8 | 74.1 | 92.7 |
Redundancy | 7.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.1M HEPES, 13% PEG-6000, 5% isopropanol, 75mM NaAcetate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |