4GKG

Crystal structure of the S-Helix Linker

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9795, 1.07210, 1.05830
Spacegroup name	P 61 2 2
Unit cell lengths	40.960, 40.960, 302.421
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.535 - 1.695
R-factor	0.2383
Rwork	0.236
R-free	0.27590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Standard 7 amino acid helix
RMSD bond length	0.010
RMSD bond angle	1.118
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.2_869))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.613
High resolution limit [Å]	1.695
Number of reflections	18052

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.2	293.15	8-12% PEG1500, 15-20% Isopropanol, 100mM Sodium Citrate, pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K
1	VAPOR DIFFUSION, SITTING DROP	5.2	293.15	8-12% PEG1500, 15-20% Isopropanol, 100mM Sodium Citrate, pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K