4GKC
Crystal structure of Q108K:K40L:T51V:T53C:R58W:T29L:Y19W:Q4A mutant of cellular retinol binding protein II complex with all-trans-retinal at 1.33
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 200 |
Detector technology | CCD |
Detector | MAR300 |
Wavelength(s) | 1.1272 |
Spacegroup name | P 1 |
Unit cell lengths | 30.970, 36.002, 64.016 |
Unit cell angles | 86.10, 86.46, 65.32 |
Refinement procedure
Resolution | 28.120 - 1.300 |
R-factor | 0.18897 |
Rwork | 0.187 |
R-free | 0.22493 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2rcq |
RMSD bond length | 0.029 |
RMSD bond angle | 2.491 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 28.122 |
High resolution limit [Å] | 1.300 |
Number of reflections | 50433 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 4.6 | 298 | 40% PEG 4000, 0.1M CH3COONa.3H2O, 0.1 M CH3COONH4, EVAPORATION, temperature 298K, pH 4.6 |