4GJZ
JMJD5 in complex with 2-oxoglutarate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-12-08 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9786 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.695, 64.056, 76.801 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.406 - 1.048 |
R-factor | 0.1475 |
Rwork | 0.147 |
R-free | 0.16280 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.312 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.3.0) |
Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 100.000 | 1.090 |
High resolution limit [Å] | 1.048 | 2.260 | 1.048 |
Rmerge | 0.089 | 0.071 | 0.453 |
Number of reflections | 110949 | ||
<I/σ(I)> | 16.4 | ||
Completeness [%] | 96.5 | 90.2 | 76.2 |
Redundancy | 12.6 | 13 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 293 | 9.5% PEG 3000, 0.1 M Bis-Tris pH 6.2, 0.05 M MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |