4GJX
Crystal structure of CD23 lectin domain mutant D258A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-01-21 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9763 |
Spacegroup name | P 65 |
Unit cell lengths | 90.970, 90.970, 351.681 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 58.670 - 2.800 |
R-factor | 0.2099 |
Rwork | 0.208 |
R-free | 0.25120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2h2r |
RMSD bond length | 0.006 |
RMSD bond angle | 0.987 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASES |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.670 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 36967 | |
Completeness [%] | 91.6 | 94.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 291 | 18% ethanol, 4% PEG 400, 0.1 M sodium acetate pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |