4GJV
Streptavidin-S112H
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-03 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0000 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 57.580, 57.580, 183.760 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.876 - 2.400 |
R-factor | 0.189 |
Rwork | 0.185 |
R-free | 0.23000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2qcb |
Data scaling software | SCALA |
Refinement software | REFMAC |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 54.946 |
High resolution limit [Å] | 2.400 |
Number of reflections | 10084 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 0.1 M MES, 19 % PEG500, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |