4GJR
Crystal structure of the TAL effector dHax3 bound to methylated dsDNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.839, 87.625, 88.455 |
| Unit cell angles | 90.00, 102.85, 90.00 |
Refinement procedure
| Resolution | 32.370 - 1.850 |
| R-factor | 0.1995 |
| Rwork | 0.198 |
| R-free | 0.22970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3v6t |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.096 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 1.850 |
| Number of reflections | 103243 |
| Completeness [%] | 99.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 291 | 10% PEG 3350, 14% ethanol, 0.1M MES pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
