4GJQ
Crystal structure of human GLTP bound with 12:0 monosulfatide (orthorhombic form;two subunits in asymmetric unit)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-03-09 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9754 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.959, 95.474, 132.390 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.000 |
R-factor | 0.2192 |
Rwork | 0.217 |
R-free | 0.26033 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3rzn |
RMSD bond length | 0.019 |
RMSD bond angle | 1.734 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.120 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 43517 | |
Completeness [%] | 98.2 | 98.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 10 % PEG 3350, 0.1M MES pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |