4GJH
Crystal Structure of the TRAF domain of TRAF5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2010-06-06 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 71.052, 90.579, 117.216 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.073 - 2.805 |
R-factor | 0.227 |
Rwork | 0.224 |
R-free | 0.27310 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.156 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MLPHARE |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.850 |
High resolution limit [Å] | 2.800 | 7.590 | 2.800 |
Number of reflections | 17854 | ||
Completeness [%] | 93.8 | 98 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 0.1M Bis-Tris, 15%(w/v) PEG10000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |