4GIP
Structure of the cleavage-activated prefusion form of the parainfluenza virus 5 (PIV5) fusion protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 250.930, 140.520, 84.600 |
| Unit cell angles | 90.00, 99.84, 90.00 |
Refinement procedure
| Resolution | 35.540 - 2.000 |
| R-factor | 0.221 |
| Rwork | 0.220 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b9b |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.137 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
| Rmerge | 0.120 | 0.053 | |
| Number of reflections | 122300 | ||
| <I/σ(I)> | 5.9 | ||
| Completeness [%] | 95.7 | 99.2 | 77.8 |
| Redundancy | 4.5 | 4.9 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 0.15 M ammonium citrate dibasic pH 5.5, 15% w/v PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
