4GIM
Crystal Structure of Pseudouridine Monophosphate Glycosidase Complexed with Pseudouridine 5'-phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-12 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9180 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.657, 76.511, 199.162 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.331 - 1.802 |
R-factor | 0.1787 |
Rwork | 0.177 |
R-free | 0.20720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vkm |
RMSD bond length | 0.007 |
RMSD bond angle | 1.222 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 3.880 | 1.800 |
Rmerge | 0.089 | 0.060 | 0.565 |
Number of reflections | 86181 | ||
<I/σ(I)> | 8.6 | ||
Completeness [%] | 99.9 | 98.8 | 100 |
Redundancy | 4.9 | 4.7 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 20% PEG4000, 0.1 M Tris, 0.2 M sodium chloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |