4GIJ

Crystal Structure of Pseudouridine Monophosphate Glycosidase Complexed with Sulfate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-17
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	62.102, 115.403, 132.017
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.443 - 1.941
R-factor	0.1868
Rwork	0.185
R-free	0.21950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vkm
RMSD bond length	0.008
RMSD bond angle	1.173
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.7.1_743)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.010
High resolution limit [Å]	1.940	4.180	1.940
Rmerge	0.056	0.032	0.385
Number of reflections	70782
<I/σ(I)>	13.8
Completeness [%]	99.7	98.9	99.6
Redundancy	5.7	5.7	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	20% PEG4000, 0.1 M Tris, 0.2 M sodium chloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K