4GIA
Crystal structure of the MUTB F164L mutant from crystals soaked with isomaltulose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-04-11 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97559 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 64.100, 86.500, 118.600 |
Unit cell angles | 90.00, 97.70, 90.00 |
Refinement procedure
Resolution | 14.950 - 2.010 |
R-factor | 0.1646 |
Rwork | 0.162 |
R-free | 0.21280 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1zjb |
RMSD bond length | 0.013 |
RMSD bond angle | 1.429 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | REFMAC |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.422 | |
Number of reflections | 84263 | |
<I/σ(I)> | 11.1 | 3.2 |
Completeness [%] | 97.1 | 81.3 |
Redundancy | 4 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 292 | 0.1M Tris-HCl, 12%(w/v) PEG20000, 0.01M L-Cysteine, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 292K |