4GI9
Crystal structure of the MUTB F164L mutant from crystals soaked with Trehalulose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97559 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.900, 85.930, 118.720 |
| Unit cell angles | 90.00, 97.92, 90.00 |
Refinement procedure
| Resolution | 14.870 - 2.150 |
| R-factor | 0.195 |
| Rwork | 0.192 |
| R-free | 0.26000 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1zjb |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.639 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.300 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.139 | 0.467 |
| Number of reflections | 66091 | |
| <I/σ(I)> | 8.3 | 2.8 |
| Completeness [%] | 95.4 | 95.4 |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 292 | 0.1M Tris-HCl, 12%(w/v) PEG20000, 0.01M L-Cysteine, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
