4GI2
Crotonyl-CoA Carboxylase/Reductase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 76 |
| Detector technology | PIXEL |
| Collection date | 2011-12-08 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.001 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 91.400, 91.400, 206.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.182 - 3.000 |
| R-factor | 0.185 |
| Rwork | 0.182 |
| R-free | 0.24080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3krt 3hzz |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.252 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 20867 | |
| Completeness [%] | 97.2 | 97.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 4M Sodium formate, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
