4GGZ
The structure of bradavidin2-biotin complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 119.067, 95.434, 49.947 |
| Unit cell angles | 90.00, 113.58, 90.00 |
Refinement procedure
| Resolution | 71.800 - 1.750 |
| R-factor | 0.19483 |
| Rwork | 0.192 |
| R-free | 0.24026 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ggr |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.670 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 71.839 |
| High resolution limit [Å] | 1.750 |
| Number of reflections | 51302 |
| Completeness [%] | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 9 | 283 | 10% PEG20000, 2% 1,4-dioxane, 0.1 M bicine, pH 9.0, LIQUID DIFFUSION, temperature 283K |
