4GGT
Structure of apo Bradavidin2 (Form B)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-04-30 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 |
Unit cell lengths | 34.771, 46.118, 46.449 |
Unit cell angles | 106.59, 106.92, 106.82 |
Refinement procedure
Resolution | 40.660 - 1.693 |
R-factor | 0.21473 |
Rwork | 0.212 |
R-free | 0.25972 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ew1 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.677 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.693 |
Number of reflections | 23010 |
Completeness [%] | 86.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 3 | 293 | 1 uL drop of 3.4 mg/mL protein in 0.5 M acetic acid, pH 3.0, with 0.12 M magnesium formate, diffraction parameters improved with later addition of 0.1 M sodium bromide, MICROBATCH, temperature 293K |