4GGR
The structure of apo bradavidin2 (Form A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-02 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.93 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 62.099, 46.061, 47.180 |
| Unit cell angles | 90.00, 116.66, 90.00 |
Refinement procedure
| Resolution | 42.170 - 1.900 |
| R-factor | 0.23799 |
| Rwork | 0.236 |
| R-free | 0.27489 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ew1 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.756 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 9133 |
| Completeness [%] | 96.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 8.5 | 293 | 30% PEG4000, 0.2 M magnesium chloride, 0.1 M Tris-HCl, pH 8.5, MICROBATCH, temperature 293K |
